Application | Comment | Organism |
---|---|---|
synthesis | coexpression with Bacillus megaterium glucose dehydrogenase in Escherichia coli for the production of L-tert-leucine. A decagram preparation of L-tert-leucine is performed at a substrate concentration of 0.6 M in 1 l scale with 99% conversion after 5.5 h, resulting in 80.1% yield and > 99% enantiomeric excess | Exiguobacterium sibiricum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Exiguobacterium sibiricum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, no residual activity | Exiguobacterium sibiricum | |
Cu2+ | 1 mM, 58.7% residual activity | Exiguobacterium sibiricum | |
Fe3+ | 1 mM, no residual activity | Exiguobacterium sibiricum | |
Mn2+ | 1 mM, 32.7% residual activity | Exiguobacterium sibiricum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
NADH | pH 9.5, 30°C | Exiguobacterium sibiricum | |
0.49 | - |
NAD+ | pH 9.5, 30°C | Exiguobacterium sibiricum | |
0.8 | - |
L-leucine | pH 9.5, 30°C | Exiguobacterium sibiricum | |
16.8 | - |
3,3-dimethyl-2-oxobutanoate | pH 9.5, 30°C | Exiguobacterium sibiricum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Exiguobacterium sibiricum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxophenylacetic acid + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | 2-aminophenylacetic acid + H2O + NAD+ | - |
r | |
3,3-dimethyl-2-oxobutanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-tert-Leu + H2O + NAD+ | - |
r | |
3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-valine + H2O + NAD+ | - |
r | |
4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-leucine + H2O + NAD+ | - |
r | |
L-isoleucine + H2O + NAD+ | - |
Exiguobacterium sibiricum | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | - |
Exiguobacterium sibiricum | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Exiguobacterium sibiricum | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 11 h | Exiguobacterium sibiricum |
60 | - |
half-life 3.1 h | Exiguobacterium sibiricum |